Dataset Information
Kinetic and sequence-structure-function analysis of known LinA variants with different hexachlorocyclohexane isomers.
Ontology highlight
ABSTRACT: Background
Here we report specific activities of all seven naturally occurring LinA variants towards three different isomers, α, γ and δ, of a priority persistent pollutant, hexachlorocyclohexane (HCH). Sequence-structure-function differences contributing to the differences in their stereospecificity for α-, γ-, and δ-HCH and enantiospecificity for (+)- and (-)-α -HCH are also discussed.Methodology/principal findings
Enzyme kinetic studies were performed with purified LinA variants. Models of LinA2(B90A) A110T, A111C, A110T/A111C and LinA1(B90A) were constructed using the FoldX computer algorithm. Turnover rates (min(-1)) showed that the LinAs exhibited differential substrate affinity amongst the four HCH isomers tested. α-HCH was found to be the most preferred substrate by all LinA's, followed by the γ and then δ isomer.Conclusions/significance
The kinetic observations suggest that LinA-γ1-7 is the best variant for developing an enzyme-based bioremediation technology for HCH. The majority of the sequence variation in the various linA genes that have been isolated is not neutral, but alters the enantio- and stereoselectivity of the encoded proteins.
SUBMITTER: Sharma P
PROVIDER: S-EPMC3174995 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Publications
Kinetic and sequence-structure-function analysis of known LinA variants with different hexachlorocyclohexane isomers.
PloS one 20110916 9
<h4>Background</h4>Here we report specific activities of all seven naturally occurring LinA variants towards three different isomers, α, γ and δ, of a priority persistent pollutant, hexachlorocyclohexane (HCH). Sequence-structure-function differences contributing to the differences in their stereospecificity for α-, γ-, and δ-HCH and enantiospecificity for (+)- and (-)-α -HCH are also discussed.<h4>Methodology/principal findings</h4>Enzyme kinetic studies were performed with purified LinA varian ...[more]
