Visualization of mRNA localization in Xenopus oocytes.
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ABSTRACT:
SUBMITTER: Gagnon JA
PROVIDER: S-EPMC3181151 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Publications
Visualization of mRNA localization in Xenopus oocytes.
Gagnon James A JA Mowry Kimberly L KL
Methods in molecular biology (Clifton, N.J.) 20110101
Visualization of in vivo mRNA localization provides a tool for understanding steps in the mechanism of transport. Here we detail a method of fluorescently labeling mRNA transcripts and microinjecting them into Xenopus laevis oocytes followed with imaging by confocal microscopy. This technique overcomes a significant hurdle of imaging RNA in the frog oocyte while providing a rapid method of visualizing mRNA localization in high resolution. ...[more]
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