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Iridium-based probe for luminescent nitric oxide monitoring in live cells.

ABSTRACT: Nitric oxide (NO) is an intra- and extracellular messenger with important functions during human physiology process. A long-lived luminescent iridium(III) complex probe 1 has been designed and synthesized for the monitoring of NO controllably released from sodium nitroprusside (SNP). Probe 1 displayed a 15-fold switch-on luminescence in the presence of SNP at 580?nm. The probe exhibited a linear response towards SNP between 5 to 25??M with detection limit at 0.18??M. Importantly, the luminescent switch-on detection of NO in HeLa cells was demonstrated. Overall, complex 1 has the potential to be applied for NO tracing in complicated cellular environment.

SUBMITTER: Wu C

PROVIDER: S-EPMC6102254 | biostudies-other | 2018 Aug

REPOSITORIES: biostudies-other

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Iridium-based probe for luminescent nitric oxide monitoring in live cells.

Wu Chun C Wu Ke-Jia KJ Kang Tian-Shu TS Wang Hui-Min David HD Leung Chung-Hang CH Liu Jin-Biao JB Ma Dik-Lung DL

Scientific reports 20180820 1

Nitric oxide (NO) is an intra- and extracellular messenger with important functions during human physiology process. A long-lived luminescent iridium(III) complex probe 1 has been designed and synthesized for the monitoring of NO controllably released from sodium nitroprusside (SNP). Probe 1 displayed a 15-fold switch-on luminescence in the presence of SNP at 580 nm. The probe exhibited a linear response towards SNP between 5 to 25 μM with detection limit at 0.18 μM. Importantly, the luminescent ...[more]

PMID: 30127525

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