Rapid high-resolution mapping of balanced chromosomal rearrangements on tiling CGH arrays.

ABSTRACT: The diagnosis and classification of many cancers depends in part on the identification of large-scale genomic aberrations such as chromosomal deletions, duplications, and balanced translocations. Array-based comparative genomic hybridization (array CGH) can detect chromosomal imbalances on a genome-wide scale but cannot reliably identify balanced chromosomal rearrangements. We describe a simple modification of array CGH that enables simultaneous identification of recurrent balanced rearrangements and genomic imbalances on the same microarray. Using custom tiling oligonucleotide arrays and gene-specific linear amplification primers, translocation CGH (tCGH) maps balanced rearrangements to ∼100-base resolution and facilitates the rapid cloning and sequencing of novel rearrangement breakpoint

SUBMITTER: Greisman HA 

PROVIDER: S-EPMC3194053 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature