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Lipid molecules influence early stages of yeast SNARE-mediated membrane fusion.

ABSTRACT: Lipid molecules, structural components of biomembranes, have been proposed for an important role in membrane fusion. Through various techniques based on a protein-reconstituted vesicle-vesicle fusion system, we investigated the influence of several lipid molecules on different stages of a yeast soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)-mediated membrane fusion process. Lipid compositions played a significant role in the early stages, docking and lipid mixing, while only exhibiting a minor effect on fusion pore formation and dilation phases, indicated by both small and large content mixing.

SUBMITTER: Lai Y

PROVIDER: S-EPMC4827429 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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Lipid molecules influence early stages of yeast SNARE-mediated membrane fusion.

Lai Ying Y Zhao Lin L Bu Bing B Lou Xiaochu X Li Dechang D Ji Baohua B Liu Jiankang J Diao Jiajie J Shin Yeon-Kyun YK

Physical biology 20150421 2

Lipid molecules, structural components of biomembranes, have been proposed for an important role in membrane fusion. Through various techniques based on a protein-reconstituted vesicle-vesicle fusion system, we investigated the influence of several lipid molecules on different stages of a yeast soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)-mediated membrane fusion process. Lipid compositions played a significant role in the early stages, docking and lipid mixing, ...[more]

PMID: 25898400

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