Ontology highlight
ABSTRACT:
SUBMITTER: Chuan P
PROVIDER: S-EPMC3200311 | biostudies-literature | 2011 Jan
REPOSITORIES: biostudies-literature
Chuan Peiying P Spudich James A JA Dunn Alexander R AR
Journal of molecular biology 20101021 1
Myosin VI is a molecular motor that is thought to function both as a transporter and as a cytoskeletal anchor in vivo. Here we use optical tweezers to examine force generation by single molecules of myosin VI under physiological nucleotide concentrations. We find that myosin VI is an efficient transporter at loads of up to ∼2 pN but acts as a cytoskeletal anchor at higher loads. Our data and the resulting model are consistent with an indirect coupling of global structural motions to nucleotide b ...[more]