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Chronic optical access through a polished and reinforced thinned skull.

ABSTRACT: We present a method to form an optical window in the mouse skull that spans millimeters and is stable for months without causing brain inflammation. This enabled us to repeatedly image blood flow in cortical capillaries of awake mice and determine long-range correlations in speed. We also repeatedly imaged dendritic spines, microglia and angioarchitecture, as well as used illumination to drive motor output via optogenetics and induce microstrokes via photosensitizers.

SUBMITTER: Drew PJ

PROVIDER: S-EPMC3204312 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature

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Chronic optical access through a polished and reinforced thinned skull.

Drew Patrick J PJ Shih Andy Y AY Driscoll Jonathan D JD Knutsen Per Magne PM Blinder Pablo P Davalos Dimitrios D Akassoglou Katerina K Tsai Philbert S PS Kleinfeld David D

Nature methods 20101021 12

We present a method to form an optical window in the mouse skull that spans millimeters and is stable for months without causing brain inflammation. This enabled us to repeatedly image blood flow in cortical capillaries of awake mice and determine long-range correlations in speed. We also repeatedly imaged dendritic spines, microglia and angioarchitecture, as well as used illumination to drive motor output via optogenetics and induce microstrokes via photosensitizers. ...[more]

PMID: 20966916

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