Ontology highlight
ABSTRACT:
SUBMITTER: Steinzeig A
PROVIDER: S-EPMC5559068 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Steinzeig Anna A Molotkov Dmitry D Castrén Eero E
PloS one 20170816 8
Growing interest in long-term visualization of cortical structure and function requires methods that allow observation of an intact cortex in longitudinal imaging studies. Here we describe a detailed protocol for the "transparent skull" (TS) preparation based on skull clearing with cyanoacrylate, which is applicable for long-term imaging through the intact skull in mice. We characterized the properties of the TS in imaging of intrinsic optical signals and compared them with the more conventional ...[more]