An alternative polyadenylation signal in TCF7L2 generates isoforms that inhibit T cell factor/lymphoid-enhancer factor (TCF/LEF)-dependent target genes.

ABSTRACT:

Aims/hypothesis

Intronic single nucleotide polymorphisms within the transcription factor 7-like 2 (TCF7L2) gene are associated with risk of type 2 diabetes. It is widely hypothesised that the predisposing variation is involved in cis-regulation of TCF7L2 activity. The aim of this study was to seek evidence for the existence of novel TCF7L2 isoforms encoded within the type 2 diabetes-associated genomic region.

Methods

We searched expressed sequence tag (EST) databases for novel TCF7L2 transcripts and sought to validate the function and integrity of any isoforms found using a combination of RT-PCR, western blotting and reporter gene techniques.

Results

Analysis of EST databases suggested the presence of an alternative polyadenylation site located in intron 4 of TCF7L2.

SUBMITTER: Locke JM 

PROVIDER: S-EPMC3210366 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature