Ontology highlight
ABSTRACT: Background
There are two selenophosphate synthetases (SPSs) in higher eukaryotes, SPS1 and SPS2. Of these two isotypes, only SPS2 catalyzes selenophosphate synthesis. Although SPS1 does not contain selenophosphate synthesis activity, it was found to be essential for cell growth and embryogenesis in Drosophila. The function of SPS1, however, has not been elucidated.Results
Differentially expressed genes in Drosophila SL2 cells were identified using two-way analysis of variance methods and clustered according to their temporal expression pattern. Gene ontology analysis was performed against differentially expressed genes and gene ontology terms related to vitamin B6 biosynthesis were found to be significantly affected at the early stage at which megamitochondria were not formed (day 3) after SPS1 knockdown. Interestingly, genes related to defense and amino acid metabolism were affected at a later stage (day 5) following knockdown. Levels of pyridoxal phosphate, an active form of vitamin B6, were decreased by SPS1 knockdown. Treatment of SL2 cells with an inhibitor of pyridoxal phosphate synthesis resulted in both a similar pattern of expression as that found by SPS1 knockdown and the formation of megamitochondria, the major phenotypic change observed by SPS1 knockdown.Conclusions
These results indicate that SPS1 regulates vitamin B6 synthesis, which in turn impacts various cellular systems such as amino acid metabolism, defense and other important metabolic activities.
SUBMITTER: Lee KH
PROVIDER: S-EPMC3218224 | biostudies-literature | 2011 Aug
REPOSITORIES: biostudies-literature
Lee Kwang Hee KH Shim Myoung Sup MS Kim Jin Young JY Jung Hee Kyoung HK Lee Eunji E Carlson Bradley A BA Xu Xue-Ming XM Park Jin Mo JM Hatfield Dolph L DL Park Taesung T Lee Byeong Jae BJ
BMC genomics 20110824
<h4>Background</h4>There are two selenophosphate synthetases (SPSs) in higher eukaryotes, SPS1 and SPS2. Of these two isotypes, only SPS2 catalyzes selenophosphate synthesis. Although SPS1 does not contain selenophosphate synthesis activity, it was found to be essential for cell growth and embryogenesis in Drosophila. The function of SPS1, however, has not been elucidated.<h4>Results</h4>Differentially expressed genes in Drosophila SL2 cells were identified using two-way analysis of variance met ...[more]