Glucocorticoids differentially regulate the expression of CRFR1 and CRFR2? in MIN6 insulinoma cells and rodent islets.

ABSTRACT: Urocortin 3 (Ucn 3), member of the corticotropin-releasing factor (CRF) family of peptide hormones, is released from ?-cells to potentiate insulin secretion. Ucn 3 activates the CRF type-2 receptor (CRFR2) but does not activate the type-1 receptor (CRFR1), which was recently demonstrated on ?-cells. While the direct actions of Ucn 3 on insulin secretion suggest the presence of cognate receptors within the islet microenvironment, this has not been established. Here we demonstrate that CRFR2? is expressed by MIN6 insulinoma cells and by primary mouse and human islets, with no detectable expression of CRFR2?. Furthermore, stimulation of MIN6 cells or primary mouse islets in vitro or in vivo with glucocorticoids (GCs) robustly and dose-dependently increases the expression of CRFR2?, while simultaneously inhibiting the expression of CRFR1 and incretin receptors. Luciferase reporters driven by the mouse CRFR1 or CRFR2? promoter in MIN6 cells confirm these differential effects of GCs. In contrast, GCs inhibit CRFR2? promoter activity in HEK293 cells and inhibit the expression of CRFR2? in A7r5 rat aortic smooth muscle cells and differentiated C2C12 myotubes. These findings suggest that the GC-mediated increase of CRFR2? depends on the cellular context of the islet and deviates from the GC-mediated suppression of CRFR1 and incretin receptors. Furthermore, GC-induced increases in CRFR2? expression coincide with increased Ucn 3-dependent activation of cAMP and MAPK pathways. We postulate that differential effect of GCs on the expression of CRFR1 and CRFR2? in the endocrine pancreas represent a mechanism to shift sensitivity from CRFR1 to CRFR2 ligands.

SUBMITTER: Huising MO 

PROVIDER: S-EPMC3219054 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature