Unknown

Dataset Information

0

Rapid, multiparameter profiling of cellular secretion using silicon photonic microring resonator arrays.


ABSTRACT: We have developed a silicon photonic biosensing chip capable of multiplexed protein measurements in a biomolecularly complex cell culture matrix. Using this multiplexed platform combined with fast one-step sandwich immunoassays, we perform a variety of T cell cytokine secretion studies with excellent time-to-result. Using 32-element arrays of silicon photonic microring resonators, the cytokines interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-5 (IL-5), and tumor necrosis factor alpha (TNF?) were simultaneously quantified with high accuracy in serum-containing cell media. Utilizing this cytokine panel, secretion profiles were obtained for primary human Th0, Th1, and Th2 subsets differentiated from naïve CD4+ T cells, and we show the ability to discriminate between lineage commitments at early stages of culture differentiation. We also utilize this approach to probe the temporal secretion patterns of each T cell type using real-time binding analyses for direct cytokine quantitation down to ?100 pM with just a 5 min-analysis.

SUBMITTER: Luchansky MS 

PROVIDER: S-EPMC3241882 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Rapid, multiparameter profiling of cellular secretion using silicon photonic microring resonator arrays.

Luchansky Matthew S MS   Bailey Ryan C RC  

Journal of the American Chemical Society 20111122 50


We have developed a silicon photonic biosensing chip capable of multiplexed protein measurements in a biomolecularly complex cell culture matrix. Using this multiplexed platform combined with fast one-step sandwich immunoassays, we perform a variety of T cell cytokine secretion studies with excellent time-to-result. Using 32-element arrays of silicon photonic microring resonators, the cytokines interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-5 (IL-5), and tumor necrosis factor alpha (TNF  ...[more]

Similar Datasets

| S-EPMC3546499 | biostudies-other
| S-EPMC4626792 | biostudies-literature
| S-EPMC3165060 | biostudies-literature
| S-EPMC3154298 | biostudies-literature
| S-EPMC5812356 | biostudies-literature
| S-EPMC2843522 | biostudies-literature
| S-EPMC2800857 | biostudies-literature
| S-EPMC2997171 | biostudies-literature
| S-EPMC3729447 | biostudies-literature
| S-EPMC3367093 | biostudies-literature