A cell-based protein-protein interaction method using a permuted luciferase reporter.
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ABSTRACT:
SUBMITTER: Eishingdrelo H
PROVIDER: S-EPMC3242404 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Publications
A cell-based protein-protein interaction method using a permuted luciferase reporter.
Eishingdrelo Haifeng H Cai Jidong J Weissensee Paul P Sharma Praveen P Tocci Michael J MJ Wright Paul S PS
Current chemical genomics 20111130
We have developed a novel cell-based protein-protein interaction assay method. The method relies on conversion of an inactive permuted luciferase containing a Tobacco Etch Virus protease (TEV) cleavage sequence fused onto protein (A) to an active luciferase upon interaction and cleavage by another protein (B) fused with the TEV protease. We demonstrate assay applicability for ligand-induced protein-protein interactions including G-protein coupled receptors, receptor tyrosine kinases and nuclear ...[more]
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