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Functional multiplex reporter assay using tagged Gaussia luciferase.

ABSTRACT: We have developed a multiplex reporter system to monitor multiple biological variables in real-time. The secreted Gaussia luciferase was fused to ten different epitope tags (Gluc(tag)), each expressed in different tumor cells. By immunobinding of the tags followed by Gluc(tag) detection, this system allowed the independent and real-time monitoring of mixed cell cultures in vitro and of mixed subcutaneous and intracranial tumor subpopulations in vivo.

SUBMITTER: van Rijn S

PROVIDER: S-EPMC3541509 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Functional multiplex reporter assay using tagged Gaussia luciferase.

van Rijn Sjoerd S Nilsson Jonas J Noske David P DP Vandertop W Peter WP Tannous Bakhos A BA Würdinger Thomas T

Scientific reports 20130110

We have developed a multiplex reporter system to monitor multiple biological variables in real-time. The secreted Gaussia luciferase was fused to ten different epitope tags (Gluc(tag)), each expressed in different tumor cells. By immunobinding of the tags followed by Gluc(tag) detection, this system allowed the independent and real-time monitoring of mixed cell cultures in vitro and of mixed subcutaneous and intracranial tumor subpopulations in vivo. ...[more]

PMID: 23308339

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