The structure of human 5-lipoxygenase.
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ABSTRACT:
SUBMITTER: Gilbert NC
PROVIDER: S-EPMC3245680 | biostudies-literature | 2011 Jan
REPOSITORIES: biostudies-literature
Publications
The structure of human 5-lipoxygenase.
Gilbert Nathaniel C NC Bartlett Sue G SG Waight Maria T MT Neau David B DB Boeglin William E WE Brash Alan R AR Newcomer Marcia E ME
Science (New York, N.Y.) 20110101 6014
The synthesis of both proinflammatory leukotrienes and anti-inflammatory lipoxins requires the enzyme 5-lipoxygenase (5-LOX). 5-LOX activity is short-lived, apparently in part because of an intrinsic instability of the enzyme. We identified a 5-LOX-specific destabilizing sequence that is involved in orienting the carboxyl terminus, which binds the catalytic iron. Here, we report the crystal structure at 2.4 angstrom resolution of human 5-LOX stabilized by replacement of this sequence. ...[more]
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