Ontology highlight
ABSTRACT:
SUBMITTER: Lin JW
PROVIDER: S-EPMC3246482 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Lin Jing-wen JW Annoura Takeshi T Sajid Mohammed M Chevalley-Maurel Séverine S Ramesar Jai J Klop Onny O Franke-Fayard Blandine M D BM Janse Chris J CJ Khan Shahid M SM
PloS one 20111227 12
Research on the biology of malaria parasites has greatly benefited from the application of reverse genetic technologies, in particular through the analysis of gene deletion mutants and studies on transgenic parasites that express heterologous or mutated proteins. However, transfection in Plasmodium is limited by the paucity of drug-selectable markers that hampers subsequent genetic modification of the same mutant. We report the development of a novel 'gene insertion/marker out' (GIMO) method for ...[more]