Bacterial whole-cell biosensor for glutamine with applications for quantifying and visualizing glutamine in plants.
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ABSTRACT:
SUBMITTER: Tessaro MJ
PROVIDER: S-EPMC3255742 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Publications
Bacterial whole-cell biosensor for glutamine with applications for quantifying and visualizing glutamine in plants.
Tessaro Michael J MJ Soliman Sameh S M SS Raizada Manish N MN
Applied and environmental microbiology 20111111 2
A whole-cell biosensor for glutamine (GlnLux) was constructed by transforming an Escherichia coli glutamine (Gln) auxotroph with a constitutive lux reporter gene. Measurements of Gln in plant extracts using GlnLux correlated with quantification using high-performance liquid chromatography (Spearman's r = 0.95). GlnLux permitted charge-coupled-device (CCD) imaging of Gln from whole plant organs. ...[more]
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