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Characterization of CIA-1, an Ambler class A extended-spectrum ?-lactamase from Chryseobacterium indologenes.

ABSTRACT: An Ambler class A ?-lactamase gene, bla(CIA-1), was cloned from the reference strain Chryseobacterium indologenes ATCC 29897 and expressed in Escherichia coli BL21. The bla(CIA-1) gene encodes a novel extended-spectrum ?-lactamase (ESBL) that shared 68% and 60% identities with the CGA-1 and CME-1 ?-lactamases, respectively. bla(CIA-1)-like genes were detected from clinical isolates. In addition to the metallo-?-lactamase IND of Ambler class B, C. indologenes has a class A ESBL gene, bla(CIA-1), located on the chromosome.

SUBMITTER: Matsumoto T

PROVIDER: S-EPMC3256067 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature

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Characterization of CIA-1, an Ambler class A extended-spectrum β-lactamase from Chryseobacterium indologenes.

Matsumoto Takehisa T Nagata Mika M Ishimine Nau N Kawasaki Kenji K Yamauchi Kazuyoshi K Hidaka Eiko E Kasuga Eriko E Horiuchi Kazuki K Oana Kozue K Kawakami Yoshiyuki Y Honda Takayuki T

Antimicrobial agents and chemotherapy 20111114 1

An Ambler class A β-lactamase gene, bla(CIA-1), was cloned from the reference strain Chryseobacterium indologenes ATCC 29897 and expressed in Escherichia coli BL21. The bla(CIA-1) gene encodes a novel extended-spectrum β-lactamase (ESBL) that shared 68% and 60% identities with the CGA-1 and CME-1 β-lactamases, respectively. bla(CIA-1)-like genes were detected from clinical isolates. In addition to the metallo-β-lactamase IND of Ambler class B, C. indologenes has a class A ESBL gene, bla(CIA-1), ...[more]

PMID: 22083470

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