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Chromosome-Encoded Broad-Spectrum Ambler Class A ?-Lactamase RUB-1 from Serratia rubidaea.

ABSTRACT: Whole-genome sequencing of Serratia rubidaea CIP 103234T revealed a chromosomally located Ambler class A ?-lactamase gene. The gene was cloned, and the ?-lactamase, RUB-1, was characterized. RUB-1 displayed 74% and 73% amino acid sequence identity with the GIL-1 and TEM-1 penicillinases, respectively, and its substrate profile was similar to that of the latter ?-lactamases. Analysis by 5' rapid amplification of cDNA ends revealed promoter sequences highly divergent from the Escherichia coli ?70 consensus sequence. This work further illustrates the heterogeneity of ?-lactamases among Serratia spp.

SUBMITTER: Bonnin RA

PROVIDER: S-EPMC5278729 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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Chromosome-Encoded Broad-Spectrum Ambler Class A β-Lactamase RUB-1 from Serratia rubidaea.

Bonnin Rémy A RA Didi Jennifer J Ergani Ayla A Lima Sandra S Naas Thierry T

Antimicrobial agents and chemotherapy 20170124 2

Whole-genome sequencing of Serratia rubidaea CIP 103234<sup>T</sup> revealed a chromosomally located Ambler class A β-lactamase gene. The gene was cloned, and the β-lactamase, RUB-1, was characterized. RUB-1 displayed 74% and 73% amino acid sequence identity with the GIL-1 and TEM-1 penicillinases, respectively, and its substrate profile was similar to that of the latter β-lactamases. Analysis by 5' rapid amplification of cDNA ends revealed promoter sequences highly divergent from the Escherichi ...[more]

PMID: 27956418

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