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Visualization and quantification of cardiac mitochondrial protein clusters with STED microscopy.

ABSTRACT: The visualization and quantification of mitochondria-associated proteins with high power microscopy methods is of particular interest to investigate protein architecture in this organelle. We report the usage of a custom-made STimulated Emission Depletion (STED) fluorescence nanoscope with ~30nm lateral resolution for protein mapping of Percoll-purified viable mitochondria from murine heart. Using this approach, we were able to quantify and resolve distinct protein clusters within mitochondria; specifically, cytochrome c oxidase subunit 2 is distributed in clusters of ~28nm; whereas the voltage dependent anion channel 1 displays three size distributions of ~33, ~55 and ~83nm.

SUBMITTER: Singh H

PROVIDER: S-EPMC3258335 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature

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Visualization and quantification of cardiac mitochondrial protein clusters with STED microscopy.

Singh Harpreet H Lu Rong R Rodríguez Pedro Felipe Gardeazábal PF Wu Yong Y Bopassa Jean Chrisostome JC Stefani Enrico E Toro Ligia L

Mitochondrion 20110925 2

The visualization and quantification of mitochondria-associated proteins with high power microscopy methods is of particular interest to investigate protein architecture in this organelle. We report the usage of a custom-made STimulated Emission Depletion (STED) fluorescence nanoscope with ~30nm lateral resolution for protein mapping of Percoll-purified viable mitochondria from murine heart. Using this approach, we were able to quantify and resolve distinct protein clusters within mitochondria; ...[more]

PMID: 21982778

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