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Coregulator-dependent facilitation of chromatin occupancy by GATA-1.


ABSTRACT: Coregulator recruitment by DNA-bound factors results in chromatin modification and protein-protein interactions, which regulate transcription. However, the mechanism by which the Friend of GATA (FOG) coregulator mediates GATA factor-dependent transcription is unknown. We showed previously that GATA-1 replaces GATA-2 at an upstream region of the GATA-2 locus, and that this GATA switch represses GATA-2. Genetic complementation analysis in FOG-1-null hematopoietic precursors revealed that FOG-1 is not required for establishment or maintenance of the active GATA-2 domain, but is critical for the GATA switch. Analysis of GATA factor binding to additional loci also revealed FOG-1-dependent GATA switches. Thus, FOG-1 facilitates chromatin occupancy by GATA-1 at sites bound by GATA-2. We propose that FOG-1 is a prototype of a new class of coregulators termed chromatin occupancy facilitators, which confer coregulation in certain contexts via enhancing trans-acting factor binding to chromatin in vivo.

SUBMITTER: Pal S 

PROVIDER: S-EPMC327128 | biostudies-literature | 2004 Jan

REPOSITORIES: biostudies-literature

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Coregulator-dependent facilitation of chromatin occupancy by GATA-1.

Pal Saumen S   Cantor Alan B AB   Johnson Kirby D KD   Moran Tyler B TB   Boyer Meghan E ME   Orkin Stuart H SH   Bresnick Emery H EH  

Proceedings of the National Academy of Sciences of the United States of America 20040108 4


Coregulator recruitment by DNA-bound factors results in chromatin modification and protein-protein interactions, which regulate transcription. However, the mechanism by which the Friend of GATA (FOG) coregulator mediates GATA factor-dependent transcription is unknown. We showed previously that GATA-1 replaces GATA-2 at an upstream region of the GATA-2 locus, and that this GATA switch represses GATA-2. Genetic complementation analysis in FOG-1-null hematopoietic precursors revealed that FOG-1 is  ...[more]

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