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The use of small molecule probes to study spatially separated stimulus-induced signaling pathways.

ABSTRACT: Simultaneous activation of signaling pathways requires dynamic assembly of higher-order protein complexes at the cytoplasmic domains of membrane-associated receptors in a stimulus-specific manner. Here, using the paradigm of cellular activation through cytokine and innate immune receptors, we demonstrate the proof-of-principle application of small molecule probes for the dissection of receptor-proximal signaling processes, such as activation of the transcription factor NF-?B and the protein kinase p38.

SUBMITTER: Kravchenko VV

PROVIDER: S-EPMC3288742 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature

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The use of small molecule probes to study spatially separated stimulus-induced signaling pathways.

Kravchenko Vladimir V VV Gloeckner Christian C Stowe G Neil GN Kang Young J YJ Tobias Peter S PS Mathison John C JC Ulevitch Richard J RJ Kaufmann Gunnar F GF Janda Kim D KD

Bioorganic & medicinal chemistry letters 20120114 5

Simultaneous activation of signaling pathways requires dynamic assembly of higher-order protein complexes at the cytoplasmic domains of membrane-associated receptors in a stimulus-specific manner. Here, using the paradigm of cellular activation through cytokine and innate immune receptors, we demonstrate the proof-of-principle application of small molecule probes for the dissection of receptor-proximal signaling processes, such as activation of the transcription factor NF-κB and the protein kina ...[more]

PMID: 22300658

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