Testing human sperm chemotaxis: how to detect biased motion in population assays.
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ABSTRACT:
SUBMITTER: Armon L
PROVIDER: S-EPMC3297605 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Publications
Testing human sperm chemotaxis: how to detect biased motion in population assays.
Armon Leah L Caplan S Roy SR Eisenbach Michael M Friedrich Benjamin M BM
PloS one 20120308 3
Biased motion of motile cells in a concentration gradient of a chemoattractant is frequently studied on the population level. This approach has been particularly employed in human sperm chemotactic assays, where the fraction of responsive cells is low and detection of biased motion depends on subtle differences. In these assays, statistical measures such as population odds ratios of swimming directions can be employed to infer chemotactic performance. Here, we report on an improved method to ass ...[more]
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