Expression and purification of Src-family kinases for solution NMR studies.
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ABSTRACT:
SUBMITTER: Piserchio A
PROVIDER: S-EPMC3305996 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Publications
Expression and purification of Src-family kinases for solution NMR studies.
Piserchio Andrea A Cowburn David D Ghose Ranajeet R
Methods in molecular biology (Clifton, N.J.) 20120101
NMR analyses of the structure, dynamics, and interactions of the Src family kinases (SFKs) have been hindered by the limited ability to obtain sufficient amounts of properly folded, soluble protein from bacterial expression systems, to allow these studies to be performed in an economically viable manner. In this chapter, we detail our attempts to overcome these difficulties using the catalytic domain (SrcCD) of c-Src, the prototypical SFK, as an illustrative example. We describe in detail two ge ...[more]
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