Unknown

Dataset Information

0

Identification and characterization of paramyosin from cyst wall of metacercariae implicated protective efficacy against Clonorchis sinensis infection.


ABSTRACT: Human clonorchiasis has been increasingly prevalent in recent years and results in a threat to the public health in epidemic regions, motivating current strategies of vaccines to combat Clonorchis sinensis (C. sinensis). In this study, we identified C. sinensis paramyosin (CsPmy) from the cyst wall proteins of metacercariae by proteomic approaches and characterized the expressed recombinant pET-26b-CsPmy protein (101 kDa). Bioinformatics analysis indicated that full-length sequences of paramyosin are conserved in helminthes and numerous B-cell/T-cell epitopes were predicted in amino acid sequence of CsPmy. Western blot analysis showed that CsPmy was expressed at four life stages of C. sinensis, both cyst wall proteins and soluble tegumental components could be probed by anti-CsPmy serum. Moreover, immunolocalization results revealed that CsPmy was specifically localized at cyst wall and excretory bladder of metacercaria, as well as the tegument, oral sucker and vitellarium of adult worm. Both immunoblot and immunolocalization results demonstrated that CsPmy was highly expressed at the stage of adult worm, metacercariae and cercaria, which could be supported by real-time PCR analysis. Both recombinant protein and nucleic acid of CsPmy showed strong immunogenicity in rats and induced combined Th1/Th2 immune responses, which were reflected by continuous high level of antibody titers and increased level of IgG1/IgG2a subtypes in serum. In vaccine trials, comparing with control groups, both CsPmy protein and DNA vaccine exhibited protective effect with significant worm reduction rate of 54.3% (p<0.05) and 36.1% (p<0.05), respectively. In consistence with immune responses in sera, elevated level of cytokines IFN-? and IL-4 in splenocytes suggested that CsPmy could induce combined cellular immunity and humoral immunity in host. Taken together, CsPmy could be a promising vaccine candidate in the prevention of C. sinensis regarding its high immunogenicity and surface localization.

SUBMITTER: Wang X 

PROVIDER: S-EPMC3312334 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

altmetric image

Publications

Identification and characterization of paramyosin from cyst wall of metacercariae implicated protective efficacy against Clonorchis sinensis infection.

Wang Xiaoyun X   Chen Wenjun W   Lv Xiaoli X   Tian Yanli Y   Men Jingtao J   Zhang Xifeng X   Lei Huali H   Zhou Chenhui C   Lu Fangli F   Liang Chi C   Hu Xuchu X   Xu Jin J   Wu Zhongdao Z   Li Xuerong X   Yu Xinbing X  

PloS one 20120321 3


Human clonorchiasis has been increasingly prevalent in recent years and results in a threat to the public health in epidemic regions, motivating current strategies of vaccines to combat Clonorchis sinensis (C. sinensis). In this study, we identified C. sinensis paramyosin (CsPmy) from the cyst wall proteins of metacercariae by proteomic approaches and characterized the expressed recombinant pET-26b-CsPmy protein (101 kDa). Bioinformatics analysis indicated that full-length sequences of paramyosi  ...[more]

Similar Datasets

| S-EPMC5680780 | biostudies-literature
| S-EPMC2788714 | biostudies-literature
| S-EPMC3588115 | biostudies-literature
2009-04-01 | GSE15447 | GEO
| S-EPMC9058273 | biostudies-literature
2010-05-20 | E-GEOD-15447 | biostudies-arrayexpress
| S-EPMC7223688 | biostudies-literature
| S-EPMC5747077 | biostudies-literature
| S-EPMC5842650 | biostudies-literature
2013-01-30 | E-MTAB-827 | biostudies-arrayexpress