In vivo identification of bipotential adipocyte progenitors recruited by ?3-adrenoceptor activation and high-fat feeding.

ABSTRACT: Nutritional and pharmacological stimuli can dramatically alter the cellular phenotypes in white adipose tissue (WAT). Utilizing genetic lineage tracing techniques, we demonstrate that brown adipocytes (BA) that are induced by ?3-adrenergic receptor activation in abdominal WAT arise from the proliferation and differentiation of cells expressing platelet-derived growth factor receptor alpha (PDGFR?), CD34, and Sca-1 (PDGFR?(+) cells). PDGFR?(+) cells have a unique morphology in which extended processes contact multiple cells in the tissue microenvironment. Surprisingly, these cells also give rise to white adipocytes (WA) that can comprise up to 25% of total fat cells in abdominal fat pads following 8 weeks of high-fat feeding. Isolated PDGFR?(+) cells differentiated into both BA and WA in vitro and generated WA after transplantation in vivo. The identification of PDGFR?(+) cells as bipotential adipocyte progenitors will enable further investigation of mechanisms that promote therapeutic cellular remodeling in adult WAT.

SUBMITTER: Lee YH 

PROVIDER: S-EPMC3322390 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature