Ontology highlight
ABSTRACT:
SUBMITTER: Kim HS
PROVIDER: S-EPMC3338534 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
PloS one 20120426 4
Attempts to express eukaryotic multi-spanning membrane proteins at high-levels have been generally unsuccessful. In order to investigate the cause of this limitation and gain insight into the rate limiting processes involved, we have analyzed the effect of translation levels on the expression of several human membrane proteins in Escherichia coli (E. coli). These results demonstrate that excessive translation initiation rates of membrane proteins cause a block in protein synthesis and ultimately ...[more]