Ontology highlight
ABSTRACT:
SUBMITTER: Xue Z
PROVIDER: S-EPMC3351553 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
Xue Zhihong Z Yuan Haiyan H Guo Jinhu J Liu Yi Y
Molecular cell 20120417 3
Argonaute proteins are required for the biogenesis of some small RNAs (sRNAs), including the PIWI-interacting RNAs and some microRNAs. How Argonautes mediate maturation of sRNAs independent of their slicer activity is not clear. The maturation of the Neurospora microRNA-like sRNA, milR-1, requires the Argonaute protein QDE-2, Dicer, and QIP. Here, we reconstitute this Argonaute-dependent sRNA biogenesis pathway in vitro and discover that the RNA exosome is also required for milR-1 production. Ou ...[more]