Ontology highlight
ABSTRACT:
SUBMITTER: Herman AG
PROVIDER: S-EPMC3353153 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Herman Ariel G AG Hayano Miki M Poyurovsky Masha V MV Shimada Kenichi K Skouta Rachid R Prives Carol C Stockwell Brent R BR
Cancer discovery 20110728 4
E3 ubiquitin ligases are of interest as drug targets for their ability to regulate protein stability and function. The oncogene Mdm2 is an attractive E3 ligase to target, as it is the key negative regulator of the tumor suppressor p53, which controls the transcription of genes involved in cell fate. Overexpression of Mdm2 facilitates tumorigenesis by inactivating p53, and through p53-independent oncogenic effects. We developed a high-throughput cellular Mdm2 auto-ubiquitination assay, which we u ...[more]