Identification and comparative expression analysis of interleukin 2/15 receptor ? chain in chickens infected with E. tenella.

ABSTRACT: BACKGROUND: Interleukin (IL) 2 and IL15 receptor ? chain (IL2/15R?, CD122) play critical roles in signal transduction for the biological activities of IL2 and IL15. Increased knowledge of non-mammalian IL2/15R? will enhance the understanding of IL2 and IL15 functions. METHODOLOGY/PRINCIPAL FINDINGS: [corrected] Chicken IL2/15R? (chIL2/15R?) cDNA was cloned using 5'/3'-RACE. The predicted protein sequence contained 576 amino acids and typical features of the type-I cytokine receptor family. COS-7 cells transfected with chIL2/15R? produced proteins of approximately 75 and 62.5 kDa under normal and tunicamycin-treated conditions, respectively. The genomic structure of chIL2/15R? was similar to its mammalian counterparts. chIL2/15R? transcripts were detected in the lymphoblast cell line CU205 and in normal lymphoid organs and at moderate levels in bursa samples. Expression profiles of chIL2/15R? and its related cytokines and receptors were examined in ConA-stimulated splenic lymphocytes and in ceca-tonsils of Eimeria tenella-infected chickens using quantitative real-time PCR. Expression levels of chIL2/15R?, chIL2R?, and chIL15R? were generally elevated in ceca-tonsils and ConA-activated splenic lymphocytes. However, chIL2 and chIL15 expression levels were differentially regulated between the samples. chIL2 expression was upregulated in ConA-activated splenic lymphocytes, but not in ceca-tonsils. In constrast, chIL15 expression was upregulated in ceca-tonsils, but not in ConA-activated splenic lymphocytes. CONCLUSIONS/SIGNIFICANCE: We identified an avian form of IL2/15R? and compared its gene expression pattern with those of chIL2, chIL15, chIL2R?, and chIL15R?. Our observations suggest that chIL15 and its receptors, including chIL2/15R?, play important roles in mucosal immunity to intestinal intracellular parasites such as Eimeria.

SUBMITTER: Jeong J 

PROVIDER: S-EPMC3360756 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature