Ontology highlight
ABSTRACT:
SUBMITTER: Luche RM
PROVIDER: S-EPMC3365281 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Luche Ralf M RM Enssle Joerg J Kiem Hans-Peter HP
Scientific reports 20120601
Despite significant improvements in lentivirus (LV) vector-based gene therapy there are still several safety risks using LV vectors including the potential formation of replication-competent LV particles. To address this shortcoming, we constructed a novel and safer gene transfer system using modified SIN-based LV gene transfer vectors. Central to our approach is a conditional deletion of the Ψ packaging signal after integration in the target genome. Here we demonstrate that after transduction o ...[more]