Abrogated cryptic activation of lentiviral transfer vectors.
Ontology highlight
ABSTRACT:
SUBMITTER: Luche RM
PROVIDER: S-EPMC3365281 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Publications
Abrogated cryptic activation of lentiviral transfer vectors.
Luche Ralf M RM Enssle Joerg J Kiem Hans-Peter HP
Scientific reports 20120601
Despite significant improvements in lentivirus (LV) vector-based gene therapy there are still several safety risks using LV vectors including the potential formation of replication-competent LV particles. To address this shortcoming, we constructed a novel and safer gene transfer system using modified SIN-based LV gene transfer vectors. Central to our approach is a conditional deletion of the Ψ packaging signal after integration in the target genome. Here we demonstrate that after transduction o ...[more]
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