Quantitative tracking of T cell clones after haematopoietic stem cell transplantation.
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ABSTRACT:
SUBMITTER: Mamedov IZ
PROVIDER: S-EPMC3377069 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
Publications
Quantitative tracking of T cell clones after haematopoietic stem cell transplantation.
Mamedov Ilgar Z IZ Britanova Olga V OV Bolotin Dmitriy A DA Chkalina Anna V AV Staroverov Dmitriy B DB Zvyagin Ivan V IV Kotlobay Alexey A AA Turchaninova Maria A MA Fedorenko Denis A DA Novik Andrew A AA Sharonov George V GV Lukyanov Sergey S Chudakov Dmitriy M DM Lebedev Yuri B YB
EMBO molecular medicine 20110304 4
Autologous haematopoietic stem cell transplantation is highly efficient for the treatment of systemic autoimmune diseases, but its consequences for the immune system remain poorly understood. Here, we describe an optimized RNA-based technology for unbiased amplification of T cell receptor beta-chain libraries and use it to perform the first detailed, quantitative tracking of T cell clones during 10 months after transplantation. We show that multiple clones survive the procedure, contribute to th ...[more]
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