Unknown

Dataset Information

0

Prediction and identification of T cell epitopes in the H5N1 influenza virus nucleoprotein in chicken.


ABSTRACT: T cell epitopes can be used for the accurate monitoring of avian influenza virus (AIV) immune responses and the rational design of vaccines. No T cell epitopes have been previously identified in the H5N1 AIV virus nucleoprotein (NP) in chickens. For the first time, this study used homology modelling techniques to construct three-dimensional structures of the peptide-binding domains of chicken MHC class ? molecules for four commonly encountered unique haplotypes, i.e., B4, B12, B15, and B19. H5N1 AIV NP was computationally parsed into octapeptides or nonapeptides according to the peptide-binding motifs of MHC class I molecules of the B4, B12, B15 and B19 haplotypes. Seventy-five peptide sequences were modelled and their MHC class I molecule-binding abilities were analysed by molecular docking. Twenty-five peptides (Ten for B4, six for B12, two for B15, and seven for B19) were predicted to be potential T cell epitopes in chicken. Nine of these peptides and one unrelated peptide were manually synthesized and their T cell responses were tested in vitro. Spleen lymphocytes were collected from SPF chickens that had been immunised with a NP-expression plasmid, pCAGGS-NP, and they were stimulated using the synthesized peptides. The secretion of chicken IFN-? and the proliferation of CD8(+) T cells were tested using an ELISA kit and flow cytometry, respectively. The significant secretion of chicken IFN-? and proliferation of CD8(+) T lymphocytes increased by 13.7% and 11.9% were monitored in cells stimulated with peptides NP(89-97) and NP(198-206), respectively. The results indicate that peptides NP(89-97) (PKKTGGPIY) and NP(198-206) (KRGINDRNF) are NP T cell epitopes in chicken of certain haplotypes. The method used in this investigation is applicable to predicting T cell epitopes for other antigens in chicken, while this study also extends our understanding of the mechanisms of the immune response to AIV in chicken.

SUBMITTER: Hou Y 

PROVIDER: S-EPMC3379973 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

altmetric image

Publications

Prediction and identification of T cell epitopes in the H5N1 influenza virus nucleoprotein in chicken.

Hou Yanxia Y   Guo Yingying Y   Wu Chunyan C   Shen Nan N   Jiang Yongping Y   Wang Jingfei J  

PloS one 20120620 6


T cell epitopes can be used for the accurate monitoring of avian influenza virus (AIV) immune responses and the rational design of vaccines. No T cell epitopes have been previously identified in the H5N1 AIV virus nucleoprotein (NP) in chickens. For the first time, this study used homology modelling techniques to construct three-dimensional structures of the peptide-binding domains of chicken MHC class Ι molecules for four commonly encountered unique haplotypes, i.e., B4, B12, B15, and B19. H5N1  ...[more]

Similar Datasets

| S-EPMC7955671 | biostudies-literature
| S-EPMC3285639 | biostudies-literature
2022-03-02 | PXD010358 | Pride
| S-EPMC4051576 | biostudies-literature
| S-EPMC2537428 | biostudies-literature
| S-EPMC7114202 | biostudies-literature
2024-05-22 | PXD035241 | Pride
2012-01-14 | E-MEXP-3432 | biostudies-arrayexpress
| S-EPMC4069037 | biostudies-literature
| S-EPMC8772545 | biostudies-literature