Proteomics

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Identification of naturally-presented influenza A virus CD8+ T cell epitopes


ABSTRACT: Influenza viruses (IV) infection is a public health concern worldwide. Currently, all available vaccines as well as antiviral drugs that target the virus itself are prone to resistance. Lessons learned from previous pandemic outbreaks is that people with a preexisting cellular immune response are either protected or developed less severe disease against the infection. Understanding CD8+ T cell immunity to IV across prominent HLA types is pivotal to rationally designing a universal influenza vaccine and generating protective immunity, especially for high-risk populations. Using mass spectroscopy, an immunopeptidomics analysis was carried out to characterize the entire landscape of peptides presented by MHC-I molecules post IV infection at different time points. We were able to identify sixteen naturally-presented MHC-I ligands restricted to different HLA allotypes including HLA-A*68, HLA-A*24, HLA-B*51, and HLA-B*07. Of these, nine peptides were immunogenic with multifunctional memory CD8 T cell response in different donors. These results highlight novel broadly protective IAV-derived CD8+ epitopes that reflect physiological peptide processing and presentation mechanisms that can work cooperatively with other distinct epitopes to provide optimal protection in particular against the newly-emerging variants.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Influenza

SUBMITTER: Hazem Ewess  

LAB HEAD: Prof. Dr. Oliver Planz

PROVIDER: PXD035241 | Pride | 2024-05-22

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Calu-3_Control_Rep_1.msf Msf
Calu-3_Control_Rep_1.raw Raw
Calu-3_Control_Rep_2.msf Msf
Calu-3_Control_Rep_2.raw Raw
Calu-3_Control_Rep_3.msf Msf
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Publications

Identification and relative abundance of naturally presented and cross-reactive influenza A virus MHC class I-restricted T cell epitopes.

Hamza Hazem H   Ghosh Michael M   Löffler Markus W MW   Rammensee Hans-Georg HG   Planz Oliver O  

Emerging microbes & infections 20240208 1


Cytotoxic T lymphocytes are key for controlling viral infection. Unravelling CD8<sup>+</sup> T cell-mediated immunity to distinct influenza virus strains and subtypes across prominent HLA types is relevant for combating seasonal infections and emerging new variants. Using an immunopeptidomics approach, naturally presented influenza A virus-derived ligands restricted to HLA-A*24:02, HLA-A*68:01, HLA-B*07:02, and HLA-B*51:01 molecules were identified. Functional characterization revealed multifunc  ...[more]

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