Ontology highlight
ABSTRACT:
SUBMITTER: Beck BD
PROVIDER: S-EPMC3388547 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Beck Brian D BD Lee Sung-Sook SS Williamson Elizabeth E Hromas Robert A RA Lee Suk-Hee SH
Biochemistry 20110427 20
Metnase (SETMAR) is a SET-transposase fusion protein that promotes nonhomologous end joining (NHEJ) repair in humans. Although both SET and the transposase domains were necessary for its function in DSB repair, it is not clear what specific role Metnase plays in the NHEJ. In this study, we show that Metnase possesses a unique endonuclease activity that preferentially acts on ssDNA and ssDNA-overhang of a partial duplex DNA. Cell extracts lacking Metnase poorly supported DNA end joining, and addi ...[more]