Ontology highlight
ABSTRACT:
SUBMITTER: Mosley RT
PROVIDER: S-EPMC3393583 | biostudies-literature | 2012 Jun
REPOSITORIES: biostudies-literature
Mosley Ralph T RT Edwards Thomas E TE Murakami Eisuke E Lam Angela M AM Grice Rena L RL Du Jinfa J Sofia Michael J MJ Furman Philip A PA Otto Michael J MJ
Journal of virology 20120411 12
The replication of the hepatitis C viral (HCV) genome is accomplished by the NS5B RNA-dependent RNA polymerase (RdRp), for which mechanistic understanding and structure-guided drug design efforts have been hampered by its propensity to crystallize in a closed, polymerization-incompetent state. The removal of an autoinhibitory β-hairpin loop from genotype 2a HCV NS5B increases de novo RNA synthesis by >100-fold, promotes RNA binding, and facilitated the determination of the first crystallographic ...[more]