Ontology highlight
ABSTRACT:
SUBMITTER: Reich S
PROVIDER: S-EPMC4230328 | biostudies-literature | 2014 Nov
REPOSITORIES: biostudies-literature
Reich Stefan S Kovermann Michael M Lilie Hauke H Knick Paul P Geissler René R Golbik Ralph Peter RP Balbach Jochen J Behrens Sven-Erik SE
Biochemistry 20141031 44
The hepatitis C virus (HCV) RNA-dependent RNA polymerase NS5B is a central enzyme of the intracellular replication of the viral (+)RNA genome. Here, we studied the individual steps of NS5B-catalyzed RNA synthesis by a combination of biophysical methods, including real-time 1D (1)H NMR spectroscopy. NS5B was found to bind to a nonstructured and a structured RNA template in different modes. Following NTP binding and conversion to the catalysis-competent ternary complex, the polymerase revealed an ...[more]