Ontology highlight
ABSTRACT:
SUBMITTER: Klein F
PROVIDER: S-EPMC3409500 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Klein Florian F Gaebler Christian C Mouquet Hugo H Sather D Noah DN Lehmann Clara C Scheid Johannes F JF Kraft Zane Z Liu Yan Y Pietzsch John J Hurley Arlene A Poignard Pascal P Feizi Ten T Morris Lynn L Walker Bruce D BD Fätkenheuer Gerd G Seaman Michael S MS Stamatatos Leonidas L Nussenzweig Michel C MC
The Journal of experimental medicine 20120723 8
Two to three years after infection, a fraction of HIV-1-infected individuals develop serologic activity that neutralizes most viral isolates. Broadly neutralizing antibodies that recognize the HIV-1 envelope protein have been isolated from these patients by single-cell sorting and by neutralization screens. Here, we report a new method for anti-HIV-1 antibody isolation based on capturing single B cells that recognize the HIV-1 envelope protein expressed on the surface of transfected cells. Altho ...[more]