Polycistronic coexpression and nondenaturing purification of histone octamers.
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ABSTRACT:
SUBMITTER: Shim Y
PROVIDER: S-EPMC3412673 | biostudies-literature | 2012 Aug
REPOSITORIES: biostudies-literature
Publications
Polycistronic coexpression and nondenaturing purification of histone octamers.
Shim Yoonjung Y Duan Ming-Rui MR Chen Xuejing X Smerdon Michael J MJ Min Jung-Hyun JH
Analytical biochemistry 20120519 2
Histone octamers are the basic building blocks of chromatin and are platforms for diverse genetic mechanisms. We report a simple method for preparing recombinant histone octamers by overexpressing all four histones from a single polycistronic vector followed by standard chromatography under native conditions. This approach reduces the time needed for the octamer preparation to a single day and should be applicable to making a variety of unmodified and modified histone octamers. ...[more]
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