Time-gated luminescence microscopy with responsive nonmetal probes for mapping activity of protein kinases in living cells.
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ABSTRACT:
SUBMITTER: Vaasa A
PROVIDER: S-EPMC3415249 | biostudies-literature | 2012 Sep
REPOSITORIES: biostudies-literature
Publications
Time-gated luminescence microscopy with responsive nonmetal probes for mapping activity of protein kinases in living cells.
Vaasa Angela A Ligi Kadri K Mohandessi Shabnam S Enkvist Erki E Uri Asko A Miller Lawrence W LW
Chemical communications (Cambridge, England) 20120720 68
A photoluminescence probe ARC-1185, possessing both high affinity towards basophilic protein kinases (PKs) and microsecond-scale luminescence lifetime when associated with a kinase, was used for the mapping of ARC-1185-PK complexes in living cells with time-gated luminescence microscopy. ...[more]
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