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Construction of covalent membrane protein complexes and high-throughput selection of membrane mimics.

ABSTRACT: The association of transmembrane (TM) helices underlies membrane protein structure and folding. Structural studies of TM complexes are limited by complex stability and the often time-consuming selection of suitable membrane mimics. Here, methodology for the efficient, preparative scale construction of covalent TM complexes and the concomitant high-throughput selection of membrane mimics is introduced. For the employed integrin ?IIb?3 model system, the methodology identified phospholipid bicelles, including their specific composition, as the best membrane mimic. The method facilitates structure determination by NMR spectroscopy as exemplified by the measurement of previously inaccessible residual dipolar couplings and (15)N relaxation parameters.

SUBMITTER: Suk JE

PROVIDER: S-EPMC3415561 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature

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Construction of covalent membrane protein complexes and high-throughput selection of membrane mimics.

Suk Jae-Eun JE Situ Alan J AJ Ulmer Tobias S TS

Journal of the American Chemical Society 20120524 22

The association of transmembrane (TM) helices underlies membrane protein structure and folding. Structural studies of TM complexes are limited by complex stability and the often time-consuming selection of suitable membrane mimics. Here, methodology for the efficient, preparative scale construction of covalent TM complexes and the concomitant high-throughput selection of membrane mimics is introduced. For the employed integrin αIIbβ3 model system, the methodology identified phospholipid bicelles ...[more]

PMID: 22626249

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