Ontology highlight
ABSTRACT:
SUBMITTER: Singh G
PROVIDER: S-EPMC3943816 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
Singh Guramrit G Ricci Emiliano P EP Moore Melissa J MJ
Methods (San Diego, Calif.) 20131002 3
Development of high-throughput approaches to map the RNA interaction sites of individual RNA binding proteins (RBPs) transcriptome-wide is rapidly transforming our understanding of post-transcriptional gene regulatory mechanisms. Here we describe a ribonucleoprotein (RNP) footprinting approach we recently developed for identifying occupancy sites of both individual RBPs and multi-subunit RNP complexes. RNA:protein immunoprecipitation in tandem (RIPiT) yields highly specific RNA footprints of cel ...[more]