Ontology highlight
ABSTRACT:
SUBMITTER: Lockard MA
PROVIDER: S-EPMC3118733 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Lockard Meghan A MA Listwan Pawel P Pedelacq Jean-Denis JD Cabantous Stéphanie S Nguyen Hau B HB Terwilliger Thomas C TC Waldo Geoffrey S GS
Protein engineering, design & selection : PEDS 20110603 7
We describe an in vitro colony screen to identify Escherichia coli expressing soluble proteins and stable, assembled multiprotein complexes. Proteins with an N-terminal 6His tag and C-terminal green fluorescent protein (GFP) S11 tag are fluorescently labeled in cells by complementation with a coexpressed GFP 1-10 fragment. After partial colony lysis, the fluorescent soluble proteins or complexes diffuse through a supporting filtration membrane and are captured on Talon(®) resin metal affinity be ...[more]