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Detection of transglutaminase activity using click chemistry.


ABSTRACT: Transglutaminase 2 (TG2) is a Ca(2+)-dependent enzyme able to catalyze the formation of ?(?-glutamyl)-lysine crosslinks between polypeptides, resulting in high molecular mass multimers. We have developed a bioorthogonal chemical method for the labeling of TG2 glutamine-donor proteins. As amine-donor substrates we used a set of azide- and alkyne-containing primary alkylamines that allow, after being crosslinked to glutamine-donor proteins, specific labeling of these proteins via the azide-alkyne cycloaddition. We demonstrate that these azide- and alkyne-functionalized TG2 substrates are cell permeable and suitable for specific labeling of TG2 glutamine-donor substrates in HeLa and Movas cells. Both the Cu(I)-catalyzed and strain promoted azide-alkyne cycloaddition proved applicable for subsequent derivatization of the TG2 substrate proteins with the desired probe. This new method for labeling TG2 substrate proteins introduces flexibility in the detection and/or purification of crosslinked proteins, allowing differential labeling of cellular proteins.

SUBMITTER: van Geel R 

PROVIDER: S-EPMC3418501 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature

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Detection of transglutaminase activity using click chemistry.

van Geel Remon R   Debets Marjoke F MF   Löwik Dennis W P M DW   Pruijn Ger J M GJ   Boelens Wilbert C WC  

Amino acids 20111217 3


Transglutaminase 2 (TG2) is a Ca(2+)-dependent enzyme able to catalyze the formation of ε(γ-glutamyl)-lysine crosslinks between polypeptides, resulting in high molecular mass multimers. We have developed a bioorthogonal chemical method for the labeling of TG2 glutamine-donor proteins. As amine-donor substrates we used a set of azide- and alkyne-containing primary alkylamines that allow, after being crosslinked to glutamine-donor proteins, specific labeling of these proteins via the azide-alkyne  ...[more]

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