Ontology highlight
ABSTRACT:
SUBMITTER: van Geel R
PROVIDER: S-EPMC3418501 | biostudies-literature | 2012 Sep
REPOSITORIES: biostudies-literature
van Geel Remon R Debets Marjoke F MF Löwik Dennis W P M DW Pruijn Ger J M GJ Boelens Wilbert C WC
Amino acids 20111217 3
Transglutaminase 2 (TG2) is a Ca(2+)-dependent enzyme able to catalyze the formation of ε(γ-glutamyl)-lysine crosslinks between polypeptides, resulting in high molecular mass multimers. We have developed a bioorthogonal chemical method for the labeling of TG2 glutamine-donor proteins. As amine-donor substrates we used a set of azide- and alkyne-containing primary alkylamines that allow, after being crosslinked to glutamine-donor proteins, specific labeling of these proteins via the azide-alkyne ...[more]