Ontology highlight
ABSTRACT:
SUBMITTER: Perez EE
PROVIDER: S-EPMC3422503 | biostudies-literature | 2008 Jul
REPOSITORIES: biostudies-literature
Nature biotechnology 20080629 7
Homozygosity for the naturally occurring Delta32 deletion in the HIV co-receptor CCR5 confers resistance to HIV-1 infection. We generated an HIV-resistant genotype de novo using engineered zinc-finger nucleases (ZFNs) to disrupt endogenous CCR5. Transient expression of CCR5 ZFNs permanently and specifically disrupted approximately 50% of CCR5 alleles in a pool of primary human CD4(+) T cells. Genetic disruption of CCR5 provided robust, stable and heritable protection against HIV-1 infection in v ...[more]