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M-Track: detecting short-lived protein-protein interactions in vivo.

ABSTRACT: We developed a protein-proximity assay in yeast based on fusing a histone lysine methyltransferase onto a bait and its substrate onto a prey. Upon binding, the prey is stably methylated and detected by methylation-specific antibodies. We applied this approach to detect varying interaction affinities among proteins in a mitogen-activated protein kinase pathway and to detect short-lived interactions between protein phosphatase 2A and its substrates that have so far escaped direct detection.

SUBMITTER: Zuzuarregui A

PROVIDER: S-EPMC3428623 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature

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M-Track: detecting short-lived protein-protein interactions in vivo.

Zuzuarregui Aurora A Kupka Thomas T Bhatt Bhumika B Dohnal Ilse I Mudrak Ingrid I Friedmann Christina C Schüchner Stefan S Frohner Ingrid E IE Ammerer Gustav G Ogris Egon E

Nature methods 20120513 6

We developed a protein-proximity assay in yeast based on fusing a histone lysine methyltransferase onto a bait and its substrate onto a prey. Upon binding, the prey is stably methylated and detected by methylation-specific antibodies. We applied this approach to detect varying interaction affinities among proteins in a mitogen-activated protein kinase pathway and to detect short-lived interactions between protein phosphatase 2A and its substrates that have so far escaped direct detection. ...[more]

PMID: 22581371

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