In vivo protein crystallization opens new routes in structural biology.
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ABSTRACT:
SUBMITTER: Koopmann R
PROVIDER: S-EPMC3429599 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Publications
In vivo protein crystallization opens new routes in structural biology.
Koopmann Rudolf R Cupelli Karolina K Redecke Lars L Nass Karol K Deponte Daniel P DP White Thomas A TA Stellato Francesco F Rehders Dirk D Liang Mengning M Andreasson Jakob J Aquila Andrew A Bajt Sasa S Barthelmess Miriam M Barty Anton A Bogan Michael J MJ Bostedt Christoph C Boutet Sébastien S Bozek John D JD Caleman Carl C Coppola Nicola N Davidsson Jan J Doak R Bruce RB Ekeberg Tomas T Epp Sascha W SW Erk Benjamin B Fleckenstein Holger H Foucar Lutz L Graafsma Heinz H Gumprecht Lars L Hajdu Janos J Hampton Christina Y CY Hartmann Andreas A Hartmann Robert R Hauser Günter G Hirsemann Helmut H Holl Peter P Hunter Mark S MS Kassemeyer Stephan S Kirian Richard A RA Lomb Lukas L Maia Filipe R N C FR Kimmel Nils N Martin Andrew V AV Messerschmidt Marc M Reich Christian C Rolles Daniel D Rudek Benedikt B Rudenko Artem A Schlichting Ilme I Schulz Joachim J Seibert M Marvin MM Shoeman Robert L RL Sierra Raymond G RG Soltau Heike H Stern Stephan S Strüder Lothar L Timneanu Nicusor N Ullrich Joachim J Wang Xiaoyu X Weidenspointner Georg G Weierstall Uwe U Williams Garth J GJ Wunderer Cornelia B CB Fromme Petra P Spence John C H JC Stehle Thilo T Chapman Henry N HN Betzel Christian C Duszenko Michael M
Nature methods 20120129 3
Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology. ...[more]
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