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Cyanine fluorophore derivatives with enhanced photostability.

ABSTRACT: Fluorescence applications requiring high photostability often depend on the use of solution additives to enhance fluorophore performance. Here we demonstrate that the direct or proximal conjugation of cyclooctatetraene (COT), 4-nitrobenzyl alcohol (NBA) or Trolox to the cyanine fluorophore Cy5 dramatically enhanced fluorophore photostability without otherwise affecting its native spectral characteristics. Such conjugation is a powerful means of improving the robustness of fluorescence-based applications demanding long-lived, nonblinking fluorescence emission.

SUBMITTER: Altman RB

PROVIDER: S-EPMC3433158 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature

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Cyanine fluorophore derivatives with enhanced photostability.

Altman Roger B RB Terry Daniel S DS Zhou Zhou Z Zheng Qinsi Q Geggier Peter P Kolster Rachel A RA Zhao Yongfang Y Javitch Jonathan A JA Warren J David JD Blanchard Scott C SC

Nature methods 20111113 1

Fluorescence applications requiring high photostability often depend on the use of solution additives to enhance fluorophore performance. Here we demonstrate that the direct or proximal conjugation of cyclooctatetraene (COT), 4-nitrobenzyl alcohol (NBA) or Trolox to the cyanine fluorophore Cy5 dramatically enhanced fluorophore photostability without otherwise affecting its native spectral characteristics. Such conjugation is a powerful means of improving the robustness of fluorescence-based appl ...[more]

PMID: 22081126

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