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Telomere-binding protein Taz1 controls global replication timing through its localization near late replication origins in fission yeast.


ABSTRACT: In eukaryotes, the replication of chromosome DNA is coordinated by a replication timing program that temporally regulates the firing of individual replication origins. However, the molecular mechanism underlying the program remains elusive. Here, we report that the telomere-binding protein Taz1 plays a crucial role in the control of replication timing in fission yeast. A DNA element located proximal to a late origin in the chromosome arm represses initiation from the origin in early S phase. Systematic deletion and substitution experiments demonstrated that two tandem telomeric repeats are essential for this repression. The telomeric repeats recruit Taz1, a counterpart of human TRF1 and TRF2, to the locus. Genome-wide analysis revealed that Taz1 regulates about half of chromosomal late origins, including those in subtelomeres. The Taz1-mediated mechanism prevents Dbf4-dependent kinase (DDK)-dependent Sld3 loading onto the origins. Our results demonstrate that the replication timing program in fission yeast uses the internal telomeric repeats and binding of Taz1.

SUBMITTER: Tazumi A 

PROVIDER: S-EPMC3444731 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature

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Telomere-binding protein Taz1 controls global replication timing through its localization near late replication origins in fission yeast.

Tazumi Atsutoshi A   Fukuura Masayoshi M   Nakato Ryuichiro R   Kishimoto Ami A   Takenaka Tomokazu T   Ogawa Shiho S   Song Ji-Hoon JH   Takahashi Tatsuro S TS   Nakagawa Takuro T   Shirahige Katsuhiko K   Masukata Hisao H  

Genes & development 20120901 18


In eukaryotes, the replication of chromosome DNA is coordinated by a replication timing program that temporally regulates the firing of individual replication origins. However, the molecular mechanism underlying the program remains elusive. Here, we report that the telomere-binding protein Taz1 plays a crucial role in the control of replication timing in fission yeast. A DNA element located proximal to a late origin in the chromosome arm represses initiation from the origin in early S phase. Sys  ...[more]

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