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Scanning angle interference microscopy reveals cell dynamics at the nanoscale.

ABSTRACT: Emerging questions in cell biology necessitate nanoscale imaging in live cells. Here we present scanning angle interference microscopy, which is capable of localizing fluorescent objects with nanoscale precision along the optical axis in motile cellular structures. We use this approach to resolve nanotopographical features of the cell membrane and cytoskeleton as well as the temporal evolution, three-dimensional architecture and nanoscale dynamics of focal adhesion complexes.

SUBMITTER: Paszek MJ

PROVIDER: S-EPMC3454456 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

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Scanning angle interference microscopy reveals cell dynamics at the nanoscale.

Paszek Matthew J MJ DuFort Christopher C CC Rubashkin Matthew G MG Davidson Michael W MW Thorn Kurt S KS Thorn Kurt S KS Liphardt Jan T JT Weaver Valerie M VM

Nature methods 20120701 8

Emerging questions in cell biology necessitate nanoscale imaging in live cells. Here we present scanning angle interference microscopy, which is capable of localizing fluorescent objects with nanoscale precision along the optical axis in motile cellular structures. We use this approach to resolve nanotopographical features of the cell membrane and cytoskeleton as well as the temporal evolution, three-dimensional architecture and nanoscale dynamics of focal adhesion complexes. ...[more]

PMID: 22751201

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